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Genome assembly workflow

This repository includes the primary steps that were used for the assembly of the topical abalone (Haliotis asinina) genome. The sequencing data for the assembly included PacBio HiFi reads (Sequel II platform), Iso-Seq (full-length cDNA using PacBio SMRT sequencing) from 5 different tissues, and Dovetail's Omni-C (proximity ligation). Please refer to the GitHub link of each tool (provided) for more info and specifications.

Main steps:

  1. Primary assembly
  2. Scaffolding
  3. Iso-Seq: genome annotation
  4. Mitochondrial genome assembly
  5. CpG methylation - Measuring DNA methylation across the genome

alt textHaliotis asinina genome