Guidescan

Guidescan is a Python-based web application and command-line tool used in genomic editing. It aids researchers in analyzing CRISPR guide RNAs for targeted gene editing. Guidescan assesses factors like on-target activity, off-target effects, and other attributes, assisting in the design of effective genome-editing experiments.

Documentation

See the Wiki for full documentation, examples, operational details and other information.

Table of Contents

• Running Guidescan in Docker
  • Prerequisites
  • Preparation
  • Generating Guidescan Data
  • Running Guidescan
• Generating Data
  • Why You Need to Read this Tutorial
  • Setting Environment Variables
  • Run Snakemake Workflow
  • Data File Structure
• Developer Setup
  • Prerequisites
  • Setting up Guidescan Environment
  • Installation
  • Data Generation
  • Configuration
  • Run Unit Tests
  • Run the Project
  • Installing guidescan (Optional)
  • Contributing to guidescanpy
• Project Structure
• Command Line Interface
  • Web
  • Decode
  • Generate Kmers
  • Initialize Database
  • Add Organism
  • Filter Tag
  • Add Tag