Cannot load pyopenms libraries to successfully generate spectral libraries in FragPipe v21.1

[jkennedy-hutch]

Good afternoon,

I have not been able to successfully fire off spectral library generation. We're running FragPipe v21.1 and have EasyPQP v0.1.42 loaded. We are getting a bunch of errors associated with OpenMS libraries, such as "ImportError: DLL load failed while importing _pyopenms_1: A dynamic link library (DLL) initialization routine failed." and "Error when loading pyOpenMS libraries! Libraries could not be found / could not be loaded." These issues are possibly due to corrupt versions of OpenSwathAlgo.dll and/or Qt5Core.dll under C:\ProgramData\Python_3.9\lib\site-packages\pyopenms. I've tried going through the Anaconda3 & OpenMS libraries to try to fix this problem, but with no luck. Any thoughts about how I can get spectral library generation going in our FragPipe?

Thanks,
-Jake

[fcyu]

Seems to be the same as #1431

The OpenMS team is working on it OpenMS/OpenMS#7333

Best,

Fengchao

[jkennedy-hutch]

Thank you very much! I’ll head over there! From: Fengchao ***@***.***> Sent: Thursday, April 18, 2024 3:52 PM To: Nesvilab/FragPipe ***@***.***> Cc: Kennedy, Jacob J ***@***.***>; Author ***@***.***> Subject: Re: [Nesvilab/FragPipe] Cannot load pyopenms libraries to successfully generate spectral libraries in FragPipe v21.1 (Issue #1543) CAUTION: This email originated from outside of the organization. Do not click links or open attachments unless you recognize the sender and know the content is safe. Seems to be the same as #1431<https://urldefense.com/v3/__https:/github.com/Nesvilab/FragPipe/issues/1431__;!!GuAItXPztq0!niBcRnZPBbUcR0Z6hAbLIfV2ABStEbYhByxpaV7EK54As177XreGHzFv4rQxSuL-cDiKN9_-yldaUvB2h1b6D6nTn5s$> The OpenMS team is working on it OpenMS/OpenMS#7333<https://urldefense.com/v3/__https:/github.com/OpenMS/OpenMS/issues/7333__;!!GuAItXPztq0!niBcRnZPBbUcR0Z6hAbLIfV2ABStEbYhByxpaV7EK54As177XreGHzFv4rQxSuL-cDiKN9_-yldaUvB2h1b618G6JJw$> Best, Fengchao — Reply to this email directly, view it on GitHub<https://urldefense.com/v3/__https:/github.com/Nesvilab/FragPipe/issues/1543*issuecomment-2065450968__;Iw!!GuAItXPztq0!niBcRnZPBbUcR0Z6hAbLIfV2ABStEbYhByxpaV7EK54As177XreGHzFv4rQxSuL-cDiKN9_-yldaUvB2h1b65qnPAZA$>, or unsubscribe<https://urldefense.com/v3/__https:/github.com/notifications/unsubscribe-auth/BH5ZB3VTYXJLT5IMTRIJ3WDY6BFB3AVCNFSM6AAAAABGODPTAOVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDANRVGQ2TAOJWHA__;!!GuAItXPztq0!niBcRnZPBbUcR0Z6hAbLIfV2ABStEbYhByxpaV7EK54As177XreGHzFv4rQxSuL-cDiKN9_-yldaUvB2h1b6IbMUKk4$>. You are receiving this because you authored the thread.Message ID: ***@***.******@***.***>>