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Really appreciate the crisprVerse team for this robust and versatile tool to visualize and annotate sgRNAs. I tried the crisprViz tool by using the example datasets provided (gpr21GuideSet and gpr21GeneModel), and it works perfectly fine, the same as in the tutorial
Now I am interested in visualizing my sgRNAs targeting a particular gene of interest. First to build a gene model, the subset of txdb_human (GRangesList) was retrieved from crisprDesignData (as mentioned in the documentation on how to build the gpr21GeneModel). This is the step-by-step of what I have tried:
Import the txdb_human (GRangesList) from crisprDesignData
Unlist the GRangesList object
Taking a subset of txdb_human by only selecting a gene and its canonical transcript (using subset function)
Create a 'type' column in the metadata to suit the required format input in makeTxDbFromGRanges
TxDb object successfully created by using makeTxDbFromGRanges function
Convert the TxDb object from step number 5 into GRangesList (the required format for plotGuideSet in crisprViz) by using TxDb2GRangesList
My plan is to directly run the plotGuideSet function after the GRangesList object is successfully created (already have the sgRNA GuideSet object). However, in the step 6, an error occurred :
> granges_list_gene_model <- TxDb2GRangesList(granges_gene_model_txdb,
+ standardChromOnly = TRUE,
+ genome = 'hg38',
+ seqlevelsStyle = 'UCSC')
Error in .getBiomartData(txdb, organism) :
Organism "NA" not recognized in biomaRt. You can use",
"organism=NULL as a solution.
I checked the genomeInfo inside the GRanges object of my gene model and compared it with the gpr21GeneModel. Both indicate the same Organism: Homo sapiens. Furthermore, I noticed the TxDb2GRangesList doesn't have a parameter to state what kind of organism the user can specify.
Looking at the source code, it turns out this function is linked with another function getTxDb which allows the user to specify the organism (default: Homo sapiens). Since specifying the organism is not a parameter in the TxDb2GRangesList function, this means the user doesn't have direct control over it. Despite it was stated that the user can use 'organism = NULL' as a solution.
Could any of the team assist in this error? in particular, the steps that I have taken so far or any other way around to resolve this issue, looking forward to hearing more soon.
The text was updated successfully, but these errors were encountered:
Really appreciate the crisprVerse team for this robust and versatile tool to visualize and annotate sgRNAs. I tried the
crisprViz
tool by using the example datasets provided (gpr21GuideSet
andgpr21GeneModel
), and it works perfectly fine, the same as in the tutorialNow I am interested in visualizing my sgRNAs targeting a particular gene of interest. First to build a gene model, the subset of
txdb_human
(GRangesList) was retrieved from crisprDesignData (as mentioned in the documentation on how to build the gpr21GeneModel). This is the step-by-step of what I have tried:makeTxDbFromGRanges
makeTxDbFromGRanges
functionTxDb2GRangesList
My plan is to directly run the plotGuideSet function after the GRangesList object is successfully created (already have the sgRNA GuideSet object). However, in the step 6, an error occurred :
I checked the
genomeInfo
inside the GRanges object of my gene model and compared it with thegpr21GeneModel
. Both indicate the same Organism: Homo sapiens. Furthermore, I noticed theTxDb2GRangesList
doesn't have a parameter to state what kind of organism the user can specify.Looking at the source code, it turns out this function is linked with another function
getTxDb
which allows the user to specify the organism (default: Homo sapiens). Since specifying the organism is not a parameter in theTxDb2GRangesList
function, this means the user doesn't have direct control over it. Despite it was stated that the user can use 'organism = NULL' as a solution.Could any of the team assist in this error? in particular, the steps that I have taken so far or any other way around to resolve this issue, looking forward to hearing more soon.
The text was updated successfully, but these errors were encountered: