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addGeneAnnotation module can not annotate the genes on the minus strand #35
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Hi all, With the help of my colleague,this bug is now fixed. When constructing the TxDb from the GFF file, I forgot to include the chrominfo parameter, which includes the chromosome length. This is necessary for the genes on the minus strand. All in all, it is a powerful tool. |
Hi all, I also want to show my script for others to use.
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Hi authors,
My studied organism is Schizosaccharomyces pombe . The latest GFF annotation file is from the Pombase website, not from Ensembl. So I construct the TxDb object by giving the GFF file. The BSgenome is also self constructed by using the BSgenome package. I want to find the spacer sequences targeting a specific gene guided by the website Design_CRISPRko_Cas9.
It is strange that the program worked very well for all genes on the plus strand, but it promotes an error for genes on the minus strand ( 'start' or 'end' cannot contain NAs).
The detailed procession is shown below.
Below are the part of two genes from gff file.
I don't know how to debug this. I am looking forward to your reply.
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