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Collapsing fails #23

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tahuh opened this issue Jul 22, 2020 · 0 comments
Open

Collapsing fails #23

tahuh opened this issue Jul 22, 2020 · 0 comments

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@tahuh
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tahuh commented Jul 22, 2020

Hi

I'm having trouble when using gencore to collapse reads (with version 0.14.0 )

I have used this tools for long time and this is the first time that I have encountered there is no collapsed read.

The original BAM file IGV image says there must exist collapsed reads as shown in IGV below

image

It seems there is an error when I see the log file and says

----Before gencore processing:
Total reads: 4014
Total bases: 581400
Mapped reads: 4014 (100.000000%)
Mapped bases: 581400 (100.000000%)
Bases mismatched with reference: 643 (0.110595%)
Reads with mismatched bases: 409 (10.189337%)
Total mapping clusters: 0
Mapping clusters with multiple fragments: 0
Total fragments: 0
Fragments with single-end reads: 0
Fragments with paired-end reads: 0
Duplication level histogram: 

----After gencore processing:
Total reads: 0
Total bases: 0
Mapped reads: 0 (Segmentation fault (core dumped)

Is there any reason for this problem?

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