diff --git a/Library/Homebrew/Gemfile b/Library/Homebrew/Gemfile
index 86fc80f2dd189..d678540fb7ac9 100644
--- a/Library/Homebrew/Gemfile
+++ b/Library/Homebrew/Gemfile
@@ -30,9 +30,6 @@ end
 group :man, optional: true do
   gem "kramdown", require: false
 end
-group :pr_upload, optional: true do
-  gem "json_schemer", require: false
-end
 group :prof, optional: true do
   gem "ruby-prof", require: false
   gem "stackprof", require: false
@@ -70,6 +67,9 @@ end
 group :audit, :bump_unversioned_casks, :livecheck, optional: true do
   gem "rexml", require: false
 end
+group :audit, :pr_upload, optional: true do
+  gem "json_schemer", require: false
+end
 
 # vendored gems (no group)
 gem "addressable"
diff --git a/Library/Homebrew/data/schemas/pypi_formula_mappings.schema.json b/Library/Homebrew/data/schemas/pypi_formula_mappings.schema.json
new file mode 100644
index 0000000000000..dbbaf765e9351
--- /dev/null
+++ b/Library/Homebrew/data/schemas/pypi_formula_mappings.schema.json
@@ -0,0 +1,55 @@
+{
+  "$id": "https://brew.sh/pypi_formula_mappings.schema.json",
+  "$schema": "https://json-schema.org/draft/2020-12/schema",
+  "title": "pypi_formula_mappings",
+  "type": "object",
+  "additionalProperties": {
+    "oneOf": [
+      { "$ref": "#/$defs/package_name" },
+      {
+        "type": "object",
+        "properties": {
+          "package_name": {
+            "description": "Normalized PyPI package name with optional extras or empty string to skip main package",
+            "type": "string",
+            "oneOf": [
+              { "$ref": "#/$defs/package_name" },
+              { "maxLength": 0 }
+            ]
+          },
+          "exclude_packages": {
+            "description": "PyPI packages to exclude from result",
+            "type": "array",
+            "items": { "$ref": "#/$defs/package_name" },
+            "minItems": 1
+          },
+          "extra_packages": {
+            "description": "PyPI packages to include with result",
+            "type": "array",
+            "items": { "$ref": "#/$defs/requirement_specifier" },
+            "minItems": 1
+          },
+          "dependencies": {
+            "description": "Formulae that need to be installed to resolve PyPI packages",
+            "type": "array",
+            "items": { "type":"string" },
+            "minItems": 1
+          }
+        },
+        "additionalProperties": false
+      }
+    ]
+  },
+  "$defs": {
+    "package_name": {
+      "description": "Normalized PyPI package name with optional extras",
+      "type": "string",
+      "pattern": "^[a-z0-9][a-z0-9-]*(\\[[a-z0-9][a-z0-9-]*(,[a-z0-9][a-z0-9-]*)*\\])?$"
+    },
+    "requirement_specifier": {
+      "description": "Normalized PyPI package name with optional extras and optional pinned version",
+      "type": "string",
+      "pattern": "^[a-z0-9][a-z0-9-]*(\\[[a-z0-9][a-z0-9-]*(,[a-z0-9][a-z0-9-]*)*\\])?(==.*)?$"
+    }
+  }
+}
diff --git a/Library/Homebrew/tap_auditor.rb b/Library/Homebrew/tap_auditor.rb
index 2e7c3cd08059b..396acd1c74e37 100644
--- a/Library/Homebrew/tap_auditor.rb
+++ b/Library/Homebrew/tap_auditor.rb
@@ -47,6 +47,15 @@ def audit_json_files
       rescue JSON::ParserError
         problem "#{file.to_s.delete_prefix("#{@path}/")} contains invalid JSON"
       end
+
+      require "json_schemer"
+      schemer = JSONSchemer.schema(HOMEBREW_DATA_PATH/"schemas/pypi_formula_mappings.schema.json")
+      return if schemer.valid?(@tap_pypi_formula_mappings)
+
+      problem <<~EOS
+        pypi_formula_mappings.json schema validation failed with following errors:
+        #{schemer.validate(@tap_pypi_formula_mappings).map { |error| "* #{error["error"]}" }.join("\n")}
+      EOS
     end
 
     sig { void }
@@ -54,6 +63,13 @@ def audit_tap_formula_lists
       check_formula_list_directory "audit_exceptions", @tap_audit_exceptions
       check_formula_list_directory "style_exceptions", @tap_style_exceptions
       check_formula_list "pypi_formula_mappings", @tap_pypi_formula_mappings
+
+      @tap_pypi_formula_mappings.each_value do |formula_mapping|
+        next unless formula_mapping.is_a?(Hash)
+        next unless formula_mapping.key?("dependencies")
+
+        check_formula_list "pypi_formula_mappings", formula_mapping["dependencies"]
+      end
     end
 
     sig { void }