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BioAlignments issue #7
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Hello,@code12412f |
@code12412f using Pkg
Pkg.status() By the way, it is a better choice to use Docker (The recommended way). The Julia environment is in a rapidly changing environment and there is a poor compatibility between package in different versions. |
Hello,
I'm getting this issue for some of my Fastq files.
[ Info: 2020-08-02 07:06:27] Program start
[ Info: 2020-08-02 07:06:28] Handing Paired-end sample: /projects/b1042/MeeksLab/abacus/6/62C51828A4DD56C3C570218C79F140DB_1_val_
1.fq.gz
[ Info: 2020-08-02 07:06:28] Aligning
[ Info: 2020-08-02 07:35:48] Reading aligned results
ERROR: LoadError: BioAlignments.SAM.Reader file format error on line 1 ~>"D00760:3"
Stacktrace:
[1] error(::Type, ::String, ::Int64, ::String, ::String) at ./error.jl:42
[2] _read!(::BioAlignments.SAM.Reader, ::BioCore.Ragel.State{BufferedStreams.BufferedInputStream{IOStream}}, ::BioAlignments.SAM.Record) at /home/kam9898/.julia/packages/BioCore/YBJvb/src/ReaderHelper.jl:164
[3] read!(::BioAlignments.SAM.Reader, ::BioAlignments.SAM.Record) at /home/kam9898/.julia/packages/BioCore/YBJvb/src/ReaderHelper.jl:134
[4] tryread!(::BioAlignments.SAM.Reader, ::BioAlignments.SAM.Record) at /home/kam9898/.julia/packages/BioCore/YBJvb/src/IO.jl:73
[5] iterate at /home/kam9898/.julia/packages/BioCore/YBJvb/src/IO.jl:84 [inlined]
[6] read_alignrs(::Dict{String,Any}, ::Array{Any,1}, ::Array{Any,1}, ::String) at /projects/b1042/MeeksLab/catt/CATT/catt.jl:230
[7] mainflow(::Dict{String,Any}, ::Array{Any,1}, ::Array{Any,1}, ::String, ::String) at /projects/b1042/MeeksLab/catt/CATT/catt.jl:700
[8] proc(::Dict{String,Any}) at /projects/b1042/MeeksLab/catt/CATT/catt.jl:764
[9] top-level scope at /projects/b1042/MeeksLab/catt/CATT/catt.jl:825
[10] include at ./boot.jl:328 [inlined]
[11] include_relative(::Module, ::String) at ./loading.jl:1105
[12] include(::Module, ::String) at ./Base.jl:31
[13] exec_options(::Base.JLOptions) at ./client.jl:287
[14] _start() at ./client.jl:460
in expression starting at /projects/b1042/MeeksLab/catt/CATT/catt.jl:824
This is the command I used:
for prefix in $(ls /projects/b1042/MeeksLab/abacus/6/62C*gz | rev | cut -c 14- | rev | uniq)
do
/projects/b1042/MeeksLab/catt/CATT/./catt --f1 ${prefix}1_val_1.fq.gz --f2 ${prefix}2_val_2.fq.gz -o $prefix -t 4
done
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