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Software Log:
nextstrain.cli 6.2.1
AUGUR 22.0.1
IQ-TREE multicore version 2.2.2.3 COVID-edition for Linux 64-bit built May 16 2023
AUSPICE 2.46.0
Genomic Epidemiology of Novel Coronavirus - Pakistan-focused Subsampling (Non-contextual/non-targeted):
Total no. of Pakistan SARS-CoV-2 Genome Sequences downloaded from GISAID (14 July 2023): 4,949
Total No. of Sequences Dropped by Nextstrain's Augur Filtering (<27000 nts): 13
562 strains were dropped during filtering
536 of these were dropped because they were in results/Pakistan/excluded_by_diagnostics.txt
13 of these were filtered out by the query: "(root_ref == 'yes' & _length >= 27000) | (pakistan == 'yes' & _length >= 27000)"
13 of these were dropped because of their ambiguous date in any
1 strains were added back because they were in defaults/include.txt
4,387 strains passed all filters
In this release, only one hCoV-19/Wuhan/WIV04/2019 (WIV04) is used as reference/root (the official reference sequence employed by GISAID; EPI_ISL_402124). WIV04 was chosen because of its high-quality genome sequence and because it represented the consensus of a handful of early submissions for the betacoronavirus responsible for COVID-19 (Pilailuk et al 2020).
Genomic Epidemiology of Novel Coronavirus - Pakistan and Related Contextual (Targeted) Samples:
Total no. of Global SARS-CoV-2 Genome Sequences downloaded from GISAID (14 July 2023): 3,716
Total no. of Pakistan SARS-CoV-2 Genome Sequences downloaded from GISAID (14 July 2023): 4,949
Total No. of Sequences that passed all filters: 2,624
In this release, only one hCoV-19/Wuhan/WIV04/2019 (WIV04) is used as reference/root (the official reference sequence employed by GISAID; EPI_ISL_402124). WIV04 was chosen because of its high-quality genome sequence and because it represented the consensus of a handful of early submissions for the betacoronavirus responsible for COVID-19 (Pilailuk et al 2020).
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v2.5.05.29.2023
OS Log:
LSB Version: core-11.1.0ubuntu2-noarch:security-11.1.0ubuntu2-noarch
Distributor ID: Ubuntu
Description: Ubuntu 20.04.5 LTS
Release: 20.04
Codename: focal
Software Log:
nextstrain.cli 6.2.1
AUGUR 22.0.1
IQ-TREE multicore version 2.2.2.3 COVID-edition for Linux 64-bit built May 16 2023
AUSPICE 2.46.0
Genomic Epidemiology of Novel Coronavirus - Pakistan-focused Subsampling (Non-contextual/non-targeted):
Total no. of Pakistan SARS-CoV-2 Genome Sequences downloaded from GISAID (14 July 2023): 4,949
Total No. of Sequences Dropped by Nextstrain's Augur Filtering (<27000 nts): 13
562 strains were dropped during filtering
536 of these were dropped because they were in results/Pakistan/excluded_by_diagnostics.txt
13 of these were filtered out by the query: "(
root_ref
== 'yes' & _length >= 27000) | (pakistan
== 'yes' & _length >= 27000)"13 of these were dropped because of their ambiguous date in any
1 strains were added back because they were in defaults/include.txt
4,387 strains passed all filters
In this release, only one hCoV-19/Wuhan/WIV04/2019 (WIV04) is used as reference/root (the official reference sequence employed by GISAID; EPI_ISL_402124). WIV04 was chosen because of its high-quality genome sequence and because it represented the consensus of a handful of early submissions for the betacoronavirus responsible for COVID-19 (Pilailuk et al 2020).
Full Change log: (https://github.com/AKU-CITRIC-Center-for-Bioinformatics/ncov/commits/v2.5)
Genomic Epidemiology of Novel Coronavirus - Pakistan and Related Contextual (Targeted) Samples:
Total no. of Global SARS-CoV-2 Genome Sequences downloaded from GISAID (14 July 2023): 3,716
Total no. of Pakistan SARS-CoV-2 Genome Sequences downloaded from GISAID (14 July 2023): 4,949
Total No. of Sequences that passed all filters: 2,624
In this release, only one hCoV-19/Wuhan/WIV04/2019 (WIV04) is used as reference/root (the official reference sequence employed by GISAID; EPI_ISL_402124). WIV04 was chosen because of its high-quality genome sequence and because it represented the consensus of a handful of early submissions for the betacoronavirus responsible for COVID-19 (Pilailuk et al 2020).
Full Change log: (https://github.com/AKU-CITRIC-Center-for-Bioinformatics/ncov/commits/v2.5)
This discussion was created from the release 07152023 - BA Contextuality in Pakistan (BA.x spinoffs).
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